Trehalose Purification
1. Feed Condition
Basic data | Feed | 3.5m3/hour；30DS (%W/W) trehalose fermentation broth |
Feed solid content | DS30 | |
Trehalose fraction | 60-65% | |
pH | 3.8-4.5 | |
Operating temperature | 55-60℃ | |
Technical indicators | Amount of resin | ~32-35m3 |
Trehalose product flow | ~6m3/hour | |
Trehalose purity | ≥90% | |
Trehalose liquid-solid content | ≥10 | |
Fructose flow | ~4.8m3/hour | |
Fructose DS | ≥10 | |
Fructose purity | ≥90% | |
Recycling water | ~13m3/hour (Trehalose balance area) |
2. Purification Process
The mixture of trehalose and glucose passes through the chromatographic separation system filled with chromatographic resin. In the resin tank, since the chromatographic resin has different forces to trehalose and glucose, trehalose flows out faster than glucose does. In addition, due to the acting force of chromatographic resin, glucose flows relatively slow and should be eluted with water. The subsequent effluent is glucose. In order to further improve the separation effect, a part of glucose solution should be mixed with the initially separated feed, and then separated through the resin in order to collect high-purity trehalose solution.
4. Operating Cost
The total annual operating cost of equipment | 177 | 10,000 yuan |
glycine output | 27720 | ton/year |
Unit processing cost | 63.8 | yuan/ton |
90%Trehalose output | 4125 | ton/year |
90%glucose output | 3300 | ton/year |
unit consumption of electricity capacity(0.7kwh) | 20.2 | yuan/ton |
Amount of processing pure water unit consumption | 42.3 | yuan/ton |
Amount of processing resin unit consumption | 1.3 | yuan/ton |
